The organisms at all trophic levels in the food chain depend on each other for food. If all organisms at a trophic level are killed, the food chain will be destroyed, all the living beings at higher trophic level will be affect by the lack of food. They will start resorting to other sources. On the other hand, organisms at lower trophic level will face sudden increase in the population which will lead to a risk of deterioration of the unbalanced ecosystem.

For example, if we kill all the deer (second trophic level), then lion, tiger and other carnivores will face difficulty with the lack of food while the plants in that region will grow at unlimited rate.