Yes, the impact of removing all the organisms in a trophic level will be different for different trophic levels. The removal of producers will affect herbivores due to absence of food. They will die. Similarly, the carnivores would also die in absence of herbivores and producers are also affected and may die due to competition for space and nutrients. Therefore, it is not possible to remove any trophic level organisms without affecting the ecosystem as they are all interlinked. This will create an imbalance in the ecosystem.